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rabbit anti rfp polyclonal  (OriGene)


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    Structured Review

    OriGene rabbit anti rfp polyclonal
    Rabbit Anti Rfp Polyclonal, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tag+polyclonal+antibody/RFP-Tag+(Ads%2E+to+Hu%2C+Ms%2C+Rt+Serum+Proteins)+Rabbit+Polyclonal+Antibody/pmc13196551-5-0-4
    Average 94 stars, based on 6 article reviews
    rabbit anti rfp polyclonal - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Immunodetection:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. For immunodetection the gels were electroblotted onto a nitrocellulose membrane (0.2 mm pore size) (Bio‐Rad) for 1 h at 100 V. The membranes were then blocked with 3% BSA (Bovine Serum Albumin, Sigma-Aldrich) in Tris buffer saline (TBS) + Tween 20 (Sigma-Aldrich) 0.1% for 1 h at RT and stained with an anti-6X His tag polyclonal antibody (OriGene). .. Detection was performed with horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad) and the enhanced chemiluminescence detection kit WesternBright ECL (Advansta) in a ChemiDoc MP Imaging System (Bio‐Rad).

    Membrane:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. For immunodetection the gels were electroblotted onto a nitrocellulose membrane (0.2 mm pore size) (Bio‐Rad) for 1 h at 100 V. The membranes were then blocked with 3% BSA (Bovine Serum Albumin, Sigma-Aldrich) in Tris buffer saline (TBS) + Tween 20 (Sigma-Aldrich) 0.1% for 1 h at RT and stained with an anti-6X His tag polyclonal antibody (OriGene). .. Detection was performed with horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad) and the enhanced chemiluminescence detection kit WesternBright ECL (Advansta) in a ChemiDoc MP Imaging System (Bio‐Rad).

    Pore Size:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. For immunodetection the gels were electroblotted onto a nitrocellulose membrane (0.2 mm pore size) (Bio‐Rad) for 1 h at 100 V. The membranes were then blocked with 3% BSA (Bovine Serum Albumin, Sigma-Aldrich) in Tris buffer saline (TBS) + Tween 20 (Sigma-Aldrich) 0.1% for 1 h at RT and stained with an anti-6X His tag polyclonal antibody (OriGene). .. Detection was performed with horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad) and the enhanced chemiluminescence detection kit WesternBright ECL (Advansta) in a ChemiDoc MP Imaging System (Bio‐Rad).

    Saline:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. For immunodetection the gels were electroblotted onto a nitrocellulose membrane (0.2 mm pore size) (Bio‐Rad) for 1 h at 100 V. The membranes were then blocked with 3% BSA (Bovine Serum Albumin, Sigma-Aldrich) in Tris buffer saline (TBS) + Tween 20 (Sigma-Aldrich) 0.1% for 1 h at RT and stained with an anti-6X His tag polyclonal antibody (OriGene). .. Detection was performed with horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad) and the enhanced chemiluminescence detection kit WesternBright ECL (Advansta) in a ChemiDoc MP Imaging System (Bio‐Rad).

    Staining:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. For immunodetection the gels were electroblotted onto a nitrocellulose membrane (0.2 mm pore size) (Bio‐Rad) for 1 h at 100 V. The membranes were then blocked with 3% BSA (Bovine Serum Albumin, Sigma-Aldrich) in Tris buffer saline (TBS) + Tween 20 (Sigma-Aldrich) 0.1% for 1 h at RT and stained with an anti-6X His tag polyclonal antibody (OriGene). .. Detection was performed with horseradish peroxidase (HRP)‐conjugated secondary antibody (Bio‐Rad) and the enhanced chemiluminescence detection kit WesternBright ECL (Advansta) in a ChemiDoc MP Imaging System (Bio‐Rad).

    Blocking Assay:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. Then, 100 μL/well of freshly prepared anti-6X His tag polyclonal antibody (OriGene) diluted in blocking solution (1:500) were added and the plates were incubated at 37°C for 1 h. Detection was obtained with goat anti-rabbit HRP secondary antibody and ABTS substrate (Roche Diagnostics). .. Absorbance at 405 nm was measured in a microplate reader (Bio-Rad).

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. Cells were washed and incubated with anti-6X His tag polyclonal antibody (OriGene) in blocking solution for 1 h at 37°C and then, with goat anti-mouse IgG1 Alexa Fluor 488 (Molecular Probes). ..

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections.
    Article Snippet: .. Then, 100 μL/well of freshly prepared anti-6X His tag polyclonal antibody (OriGene) diluted in blocking solution (1:500) were added and the plates were incubated at 37 ̊C for 1 h. Detection was obtained with goat anti-rabbit HRP secondary antibody and ABTS substrate (Roche Diagnostics). .. Absorbance at 405 nm was measured in a microplate reader (Bio-Rad).

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections.
    Article Snippet: .. Cells were washed and incubated with anti-6X His tag polyclonal antibody (OriGene) in blocking solution for 1 h at 37 ̊C and then, with goat anti-mouse IgG1 Alexa Fluor 488 (Molecular Probes). ..

    Incubation:

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. Then, 100 μL/well of freshly prepared anti-6X His tag polyclonal antibody (OriGene) diluted in blocking solution (1:500) were added and the plates were incubated at 37°C for 1 h. Detection was obtained with goat anti-rabbit HRP secondary antibody and ABTS substrate (Roche Diagnostics). .. Absorbance at 405 nm was measured in a microplate reader (Bio-Rad).

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections
    Article Snippet: .. Cells were washed and incubated with anti-6X His tag polyclonal antibody (OriGene) in blocking solution for 1 h at 37°C and then, with goat anti-mouse IgG1 Alexa Fluor 488 (Molecular Probes). ..

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections.
    Article Snippet: .. Then, 100 μL/well of freshly prepared anti-6X His tag polyclonal antibody (OriGene) diluted in blocking solution (1:500) were added and the plates were incubated at 37 ̊C for 1 h. Detection was obtained with goat anti-rabbit HRP secondary antibody and ABTS substrate (Roche Diagnostics). .. Absorbance at 405 nm was measured in a microplate reader (Bio-Rad).

    Article Title: Development and in vitro characterization of a humanized scFv against fungal infections.
    Article Snippet: .. Cells were washed and incubated with anti-6X His tag polyclonal antibody (OriGene) in blocking solution for 1 h at 37 ̊C and then, with goat anti-mouse IgG1 Alexa Fluor 488 (Molecular Probes). ..



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    Nuclear actin-YAP binding plays a pivotal role in cell proliferation. ( A–B ) AlphaFold 3 prediction model of TEAD4 (magenta) interaction with (A) YAP (cyan) or (B) YAP (cyan) and G-actin (green) in the presence of M-CAT DNA. The black box indicated the predicted four-chain α-helical interface (4α) interacts with TEAD4 DNA binding domain (DBD). ( C ) Immunoblot analysis of Lysate and IP fractions from GFP-trap co-immunoprecipitation experiment in HeLa wt cells or HeLa cells stably expressing FLAG-3X NLS-EGFP-YAP WT and transiently transfected with FLAG-NLS-actin and myc-TEAD4. ( D ) ChIP-qPCR analysis of YAP–TEAD4 recruitment to target genes in HeLa cells transfected with NLS-EGFP (pink, triangle), NLS-EGFP-YAP WT (blue, circle) and NLS-EGFP-YAP DDY (green, square). ZFP37 served as a negative control. Data are from three independent experiments. Statistical analysis was done using a two-tailed unpaired Student’s t-test ( P < 0.05, P < 0.01). ( E ) Quantitative PCR (qPCR) analysis of YAP target gene expression in HeLa cells transfected with NLS-BFP (Mock) or NLS-actin in the absence or presence of myc-YAP WT or myc-YAP DDY . Data are normalized to the NLS-BFP Mock control mRNA transcripts. Each dot represents an independent experiment. Statistical analysis was done using One-way ANOVA with Tukey´s multiple comparison test ( P < 0.05, P < 0.01, P < 0.0001). ( F ) Dose response curves of purified YAP WT (blue) and YAP DDY (green) over TEAD4-coated CM5 chip, indicating the dissociation constant (K d ). ( G ) Representative images of Click-iT® Plus EdU assay of HeLa cells transfected with empty plasmid (Mock), myc-YAP WT and myc -YAP DDY and stained <t>for</t> <t>anti-myc</t> (green), DAPI (blue) and EdU (magenta). Images are shown as MIPs, Scale bar = 20 μm. ( H ) Quantification of the ratio of EdU + cells. Data are shown as a violin plot with median, quartiles and individual data points from three independent biological replicates (green, magenta, cyan). Each dot represents the percentage of EdU + cells per field of view. Statistical analysis was done using One-way ANOVA with Tukey´s multiple comparison test ( P < 0.0001). n = 26, 24, and 24.
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    Image Search Results


    Nuclear actin-YAP binding plays a pivotal role in cell proliferation. ( A–B ) AlphaFold 3 prediction model of TEAD4 (magenta) interaction with (A) YAP (cyan) or (B) YAP (cyan) and G-actin (green) in the presence of M-CAT DNA. The black box indicated the predicted four-chain α-helical interface (4α) interacts with TEAD4 DNA binding domain (DBD). ( C ) Immunoblot analysis of Lysate and IP fractions from GFP-trap co-immunoprecipitation experiment in HeLa wt cells or HeLa cells stably expressing FLAG-3X NLS-EGFP-YAP WT and transiently transfected with FLAG-NLS-actin and myc-TEAD4. ( D ) ChIP-qPCR analysis of YAP–TEAD4 recruitment to target genes in HeLa cells transfected with NLS-EGFP (pink, triangle), NLS-EGFP-YAP WT (blue, circle) and NLS-EGFP-YAP DDY (green, square). ZFP37 served as a negative control. Data are from three independent experiments. Statistical analysis was done using a two-tailed unpaired Student’s t-test ( P < 0.05, P < 0.01). ( E ) Quantitative PCR (qPCR) analysis of YAP target gene expression in HeLa cells transfected with NLS-BFP (Mock) or NLS-actin in the absence or presence of myc-YAP WT or myc-YAP DDY . Data are normalized to the NLS-BFP Mock control mRNA transcripts. Each dot represents an independent experiment. Statistical analysis was done using One-way ANOVA with Tukey´s multiple comparison test ( P < 0.05, P < 0.01, P < 0.0001). ( F ) Dose response curves of purified YAP WT (blue) and YAP DDY (green) over TEAD4-coated CM5 chip, indicating the dissociation constant (K d ). ( G ) Representative images of Click-iT® Plus EdU assay of HeLa cells transfected with empty plasmid (Mock), myc-YAP WT and myc -YAP DDY and stained for anti-myc (green), DAPI (blue) and EdU (magenta). Images are shown as MIPs, Scale bar = 20 μm. ( H ) Quantification of the ratio of EdU + cells. Data are shown as a violin plot with median, quartiles and individual data points from three independent biological replicates (green, magenta, cyan). Each dot represents the percentage of EdU + cells per field of view. Statistical analysis was done using One-way ANOVA with Tukey´s multiple comparison test ( P < 0.0001). n = 26, 24, and 24.

    Journal: Nucleic Acids Research

    Article Title: Regulation of YAP activity by nuclear G-actin binding

    doi: 10.1093/nar/gkag248

    Figure Lengend Snippet: Nuclear actin-YAP binding plays a pivotal role in cell proliferation. ( A–B ) AlphaFold 3 prediction model of TEAD4 (magenta) interaction with (A) YAP (cyan) or (B) YAP (cyan) and G-actin (green) in the presence of M-CAT DNA. The black box indicated the predicted four-chain α-helical interface (4α) interacts with TEAD4 DNA binding domain (DBD). ( C ) Immunoblot analysis of Lysate and IP fractions from GFP-trap co-immunoprecipitation experiment in HeLa wt cells or HeLa cells stably expressing FLAG-3X NLS-EGFP-YAP WT and transiently transfected with FLAG-NLS-actin and myc-TEAD4. ( D ) ChIP-qPCR analysis of YAP–TEAD4 recruitment to target genes in HeLa cells transfected with NLS-EGFP (pink, triangle), NLS-EGFP-YAP WT (blue, circle) and NLS-EGFP-YAP DDY (green, square). ZFP37 served as a negative control. Data are from three independent experiments. Statistical analysis was done using a two-tailed unpaired Student’s t-test ( P < 0.05, P < 0.01). ( E ) Quantitative PCR (qPCR) analysis of YAP target gene expression in HeLa cells transfected with NLS-BFP (Mock) or NLS-actin in the absence or presence of myc-YAP WT or myc-YAP DDY . Data are normalized to the NLS-BFP Mock control mRNA transcripts. Each dot represents an independent experiment. Statistical analysis was done using One-way ANOVA with Tukey´s multiple comparison test ( P < 0.05, P < 0.01, P < 0.0001). ( F ) Dose response curves of purified YAP WT (blue) and YAP DDY (green) over TEAD4-coated CM5 chip, indicating the dissociation constant (K d ). ( G ) Representative images of Click-iT® Plus EdU assay of HeLa cells transfected with empty plasmid (Mock), myc-YAP WT and myc -YAP DDY and stained for anti-myc (green), DAPI (blue) and EdU (magenta). Images are shown as MIPs, Scale bar = 20 μm. ( H ) Quantification of the ratio of EdU + cells. Data are shown as a violin plot with median, quartiles and individual data points from three independent biological replicates (green, magenta, cyan). Each dot represents the percentage of EdU + cells per field of view. Statistical analysis was done using One-way ANOVA with Tukey´s multiple comparison test ( P < 0.0001). n = 26, 24, and 24.

    Article Snippet: The following primary were used: mouse monoclonal anti-FLAG® M2 antibody (1:1000, Merck, F1804), YAP (D8H1X) XP® Rabbit mAb (1:500, Cell Signaling Technology, 14 074), rabbit polyclonal anti-Myc Tag (1:1000, Cell Signaling Technology, 2272), mCherry (E5D8F) Rabbit mAb (1:1000, Cell Signaling Technology, 43 590), anti-Tubulin (11H10) Rabbit mAb (1:1000, Cell Signaling Technology, 2125), mouse monoclonal anti-Actin antibody (Merck, A4700), eGFP mouse monoclonal antibody (F56-6A1.2.3) (1:1000, Invitrogen, MA1-952), anti-GAPDH mouse mAb (6C5) (1:2000, Millipore, CB1001).

    Techniques: Binding Assay, Western Blot, Immunoprecipitation, Stable Transfection, Expressing, Transfection, ChIP-qPCR, Negative Control, Two Tailed Test, Real-time Polymerase Chain Reaction, Targeted Gene Expression, Control, Comparison, Purification, EdU Assay, Plasmid Preparation, Staining